Potato extract inhibits lipase activity and ameliorates gut microbiome dysbiosis and weight gain in mice fed a high-fat diet

Abstract Curtailing the absorption of triglycerides (TGs) is a preferred pathway for treating obesity. Our previous study demonstrated that water-soluble fraction from potato could inhibit lipase activity patatin, one major proteins in potato. This aqueous was purified and concentrated by deproteination reversed-phase chromatography to investigate effectiveness against Biochemical analyses indicated non-competitively inhibited pancreatic (PLase) with half-maximal inhibitory concentration 10.17 µg/mL, named as potato-derived (PI). Animal studies on C57BL/6 mice showed fed high-fat diet (HFD), PI treatment resulted reductions body weight gain, adipose fat deposition, liver TGs, ameliorated gut microbiome dysbiosis caused HFD feeding; meanwhile, orlistat, well-known inhibitor, diverged profile HFD. High resolution electronspray ionization-Orbitrap tandem mass spectrometry identified gallic acid, 4-hydroxybenzoic protocatechuic which are known have activities, PI. However, these compounds not reconstitute comparable specific inferring existence another compound(s) be